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monoclonal anti-Glutamate antibody

貨號 貨期 規格 / 價格 詢價

monoclonal anti-Glutamate antibody

品牌

Swant

貨號

2D7

產品分類

單克隆抗體

研究領域

Antibodies against neurotransmitters

產品概述

Store stock-solution in freezer (-20°C, not -70°C). Mix stock solution well before making aliquots. 10x diluted solutions may be kept in the refrigerator for some time (several weeks) but should be made 10 mM with sodium azide. Volumes of working dilutions (high dilutions) should be calculated such the they are used up the same day. Avoid polystyrene tubes.

形式

The antibodies have been purified from ascitic fluid by ammoniumsulfate precipitation and subsequently by FPLC on Protein-A-Superose. BSA has been added to the eluate, and the material has been dialyzed against water and lyophilized.

濃度

Glutamate

純度

Mouse monoclonal, IgG1

試劑準備

Lyophilized (no preservatives).

復溶

Liu CJ, Grandes P, Matute C, Cuenod M, Streit P: Glutamate-like immunoreactivity revealed in rat olfactory bulb, hippocampus and cerebellum by monoclonal antibody and sensitive staining method. Histochemistry 1989;90(6):427-45

應用

Appropriate final dilutions should be determined experimentally. For LM, we strongly suggest to use postembedding staining and to combine the anti-Glu antibody with a silver-enhanced PAP procedure as described in detail by Liu et al.(1989). For EM, we recommend a postembedding immunogold procedure (see: Liu et al., 1989).

產品描述

Product: Conc. supernat. Ref: 13 Code No: mAB 2D7 Lot No: ps3 Reconstitution: add the lyophilized material (approx. 1 mg) to 100 ul PBS or another buffered saline (add in order indicated) allow some time for the material to dissolve; mix by swirling gently remove particulate matter by centrifugation in a microfuge add 100 ul glycerol (final glycerol conc. 50% v/v) and mix well (vortex)

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