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Calmodulin

貨號 貨期 規格 / 價格 詢價

Calmodulin

品牌

Swant

貨號

rCM

產品分類

Recombinant Protein

研究領域

Others

產品概述

Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.

形式

This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: - Dilute 1 μl of the antibody 465 (Swant) against calmodulin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 μg of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

濃度

recombinant calmodulin produced in Escherichia Coli.

純度

VU1

試劑準備

lyophilized (10 ug in 10 ul of: 10 mM Tris-HCl, 1 mM EGTA).

產品描述

Purification: HPLC Lot No: VU-1

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數量

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